Bacillus thuringiensis is a Gram-positive soil bacterium known as biopesticide bacteria that produce insecticidal proteins called protein crystals (Cry). In the process of insecticides, chitinase advisable to perforate the membrane barrier peritrophic to facilitate the invasion of Cry proteins to the membrane epithelium. A chitinase gene from B. thuringiensis successfully expressed in soluble form in Escherichia coli and purified and characterized the gene product. Purified recombinant enzyme, BthChi74, hydrolyzed artificial substrates, 4-nitrophenyl N, N'-diacetyl-β-D-chitobioside [4NP- (GlcNAc) 2], and the natural substrate, colloidal chitin and crystal α-chitin, but it does not hydrolyze cellulose. BthChi74 shows the catalytic activity under weakly acidic to neutral pH Yeast Recombinant Proteins range at 50 ° C, and it was stable at broad pH range for 24 hours. Differential scanning Uranium (DSF) shows the protein melting temperature (T m) of 63.6 ° C. The kin...