Kinetic and oligomeric study of Leishmania braziliensis nicotinate/nicotinamide mononucleotide adenylyltransferase.
Nicotinamide adenine dinucleotide (NAD) is an essential coenzyme involved in redox reactions and oxidative stress defense system. Furthermore, the NAD is used as the substrate by a protein that regulates cell f unctions important for DNA repair, Other Recombinant Proteins genetic and signal transduction, among many others. NAD biosynthesis can be solved through de novo and salvage pathways, which converge at a common step is catalyzed by a nicotinate / nicotinamide mononucleotide adenylyltransferase (NMNAT EC: 2.7.7.1/18). Here, we report kinetic characterization of NMNAT of Leishmania braziliensis (LbNMNAT), one of the etiologic agent of leishmaniasis, a parasitic disease that is relevant. Expression and purification of recombinant proteins homogeneous 6xHis-LbNMNAT done and kinetic studies, which included analysis of Km, Vmax, kcat and equilibrium constants (KD) for both forward and reverse reactions, is completed. Oligomeric state of the recombinant protein 6xHis-LbNMNAT ...