Effect of recombinant α1-antitrypsin Fc-fused (AAT-Fc)protein on the inhibition of inflammatory cytokine production and streptozotocin-induced diabetes.
α1-Antitrypsin (AAT) is a member of a family of serine proteinase inhibitors which inhibit the activity of serine proteinase enzymes including human neutrophil elastase, cathepsin G and neutrophil proteinase 3. Here, we expr essed recombinant AAT AAT gene Hapten Recombinant Proteins intact combining to IgG1 constant region to produce soluble recombinant AAT-Fc protein. Recombinant AAT-Fc protein produced in Chinese hamster ovary (CHO) cells and purified using affinity chromatography mini-proteins A. Recombinant AAT-Fc protein was tested for anti-inflammatory function and AAT-Fc adequately suppressed tumor necrosis factor (TNF) -α-induced interleukin (IL) -6 in human peripheral blood mononuclear cells (PBMC) and inhibit cytokine-induced TNF by different cytokines in mouse Raw 264.7 macrophage cells. However, AAT-Fc failed lipopolysaccharide-induced cytokine production presses in both PBMC and macrophages. In addition, our data indicate that AAT-Fc blocks the development of h...