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Effect of recombinant α1-antitrypsin Fc-fused (AAT-Fc)protein on the inhibition of inflammatory cytokine production and streptozotocin-induced diabetes.

α1-Antitrypsin (AAT) is a member of a family of serine proteinase inhibitors which inhibit the activity of serine proteinase enzymes including human neutrophil elastase, cathepsin G and neutrophil proteinase 3. Here, we expressed recombinant AAT AAT gene Hapten Recombinant Proteins intact combining to IgG1 constant region to produce soluble recombinant AAT-Fc protein. Recombinant AAT-Fc protein produced in Chinese hamster ovary (CHO) cells and purified using affinity chromatography mini-proteins A. 

Recombinant AAT-Fc protein was tested for anti-inflammatory function and AAT-Fc adequately suppressed tumor necrosis factor (TNF) -α-induced interleukin (IL) -6 in human peripheral blood mononuclear cells (PBMC) and inhibit cytokine-induced TNF by different cytokines in mouse Raw 264.7 macrophage cells.

However, AAT-Fc failed lipopolysaccharide-induced cytokine production presses in both PBMC and macrophages. In addition, our data indicate that AAT-Fc blocks the development of hyperglycemia in a mouse model of streptozotocin-induced diabetes. Interestingly, we also found that plasma-derived AAT specifically inhibit the activity of the enzyme elastase but it AAT-Fc no inhibitory effect on elastase activity.

Effect of recombinant α1-antitrypsin Fc-fused (AAT-Fc)protein on the inhibition of inflammatory cytokine production and streptozotocin-induced diabetes.


hHscFv recombinant protein-RC-RNase derived from transgenic tobacco acts as a bifunctional molecule complexes against hepatocellular carcinoma.


hepatocellular carcinoma (HCC) is a common clinical malignant primary tumor; However, efficient drugs for the treatment of HCC is still lacking at the moment. To develop new approaches for the treatment of liver cancer, we designed a gene chimeric (her-HR) encodes a single chain variable fragment of human HAb25 (hHscFv) fused to ribonuclease cytotoxic of Rana catesbeiana (RC-RNase) and otherwise in accordance fusion protein in transgenic tobacco ( Nicotiana tabacum). 

Eleven positively identified transgenic plant lines of 204 tobacco plants regenerated by PCR and Southern blot analysis, and immunocompetence of her-HR recombinant protein was confirmed by Western blotting. HR-protein expression levels ranged from 0.75 to 1.99 ug / g in fresh tobacco leaves. 

To characterize its bifunction-HR protein Monkey Recombinant Proteins expressed in tobacco, specificity and toxicity to cells bind multiple cell lines examined by streptavidin-biotin complex method of indirect immunocytochemical and 3- (4,5-dimethylthiazol-2-yl) -2, 5- diphenyl-2H-tetrazolium bromide assay. Data show that the protein Her-HR has a special powerful affinity for HepG2 (cell line HCC human liver) than tumor cell lines other and the line of normal liver cells bind, and the capacity to kill the cell line HCC SMMC7721 and HepG2 with half-maximal inhibiting concentrations of 2, 0 and 2.4 nM, respectively
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